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KMID : 1234420080360020101
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Korean Journal of Microbiololgy and Biotechnology
2008 Volume.36 No. 2 p.101 ~ p.105
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Construction of A Bacteriocidal Yeast Producing Bacteriocin OR-7
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Lee Ok-Hee
Jang Min-Kyung
Lee Dong-Geun
Lee Jae-Hwa
Ha Jong-Myuong
Ha Bae-Jin
Ahn Ik-Yong
Cho Dong-In
Lee Sang-Hyeon
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Abstract
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In order to obtain yeast cells producing a bacteriocin OR-7, the 180 bp polynucleotide corresponding to the OR-7 gene including codons for start and stop was chemically synthesized and cloned into pAUR123, an yeast expression vector. Transformed yeast cells exhibited growth inhibition of Bacillus subtilis, Campylobacter jejuni, Escherichia coli and Pseudomonas aeruginosa. This result indicates that yeast cells producing OR-7 possess bacteriocidal properties against both Gram positive B. subtilis and Gram negative C. jejuni, E. coli and P. aeruginosa cells. The recombinant yeast strain constructed in this study can be applied in the food preservative or animal feed.
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KEYWORD
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Bacteriocin, Bacillus subtilis, Campylobacter jejuni, Escherichia coli, OR-7, Pseudomonas aeruginosa, Saccharomyces cerevisiae, yeast
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